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hilyte 647 tubulin  (Cytoskeleton Inc)


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    Cytoskeleton Inc hilyte 647 tubulin
    a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte <t>647-tubulin),</t> CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.
    Hilyte 647 Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+hilyte+647+tubulin/Tubulin+protein+-+fluorescent+HiLyte+647+porcine+brain/bio_rxiv__64898__2026__08__11__744254-295-0-2
    Average 95 stars, based on 97 article reviews
    hilyte 647 tubulin - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Local fluidization of an active cytoplasmic gel partitions large cells"

    Article Title: Local fluidization of an active cytoplasmic gel partitions large cells

    Journal: bioRxiv

    doi: 10.64898/2026.08.11.744254

    a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte 647-tubulin), CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.
    Figure Legend Snippet: a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte 647-tubulin), CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.

    Techniques Used: Immunofluorescence, Labeling, Diffusion-based Assay

    Related Articles

    other:

    Article Title: Actin and microtubule crosslinkers tune mobility and control co-localization in a composite cytoskeletal network.
    Article Snippet: Actin and microtubule filaments, with their auxiliary proteins, enable the cytoskeleton to carry out vital processes in the cell by tuning the organizational and mechanical properties of the network.. Despite their critical importance and interactions in cells, we are only beginning to uncover information about the composite network.. The challenge is due to the high complexity of combining actin, microtubules, and their hundreds of known associated proteins.

    Purification:

    Article Title: The endoplasmic reticulum protein Erg28 restrains Mto1-Mto2-γ-TuSC-mediated microtubule assembly.
    Article Snippet: .. The reaction mix, which consisted of MRB80 (80 mM PIPES, pH 6.8, 1 mM EGTA, 4 mM MgCl2), 20 μM unlabeled porcine brain tubulin (Cytoskeleton), 0.5 μM Rhodaminetubulin or Fluorescent HiLyte 647 tubulin (Cytoskeleton), 1 mM GTP, 0.2 mg/mL κ-casein, 0.1% methylcellulose, oxygen scavenger mix (50 mM glucose, 400 μg/mL glucose oxidase, 200 μg/mL catalase, and 4 mM DTT), and purified proteins (9.45 nM Mto1(bonsai)GFP-Mto2 or 12 nM Mto1(FL)-GFP-Mto2, 6.75 nM γ-TuSC, 15 nM Erg28-GFP/mCherry or 15 nM Erg31-GFP/mCherry) to a final volume of 25 μL, were centrifuged in an Air-fuge for 2.5 min at ∼28 psi (90,000 rpm, 160,000 g). ..



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    a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte <t>647-tubulin),</t> CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.
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    Image Search Results


    a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte 647-tubulin), CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.

    Journal: bioRxiv

    Article Title: Local fluidization of an active cytoplasmic gel partitions large cells

    doi: 10.64898/2026.08.11.744254

    Figure Lengend Snippet: a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte 647-tubulin), CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.

    Article Snippet: HiLyte 647-tubulin (Cytoskeleton, Inc. TL670M) and mRNAs were mixed with final concentrations of 800 ng/μL for tubulin and150 ng/μL for each mRNA.

    Techniques: Immunofluorescence, Labeling, Diffusion-based Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Cisplatin resistance in an ovarian cancer model is mediated by microtubule dynamics regulator TPPP3 in synergy with tubulin code rewiring

    doi: 10.1016/j.celrep.2026.117414

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Paclitaxel-stabilized cisplatin sensitive (CP-S) microtubules containing 97% CP-S tubulin, 1% biotin brain tubulin, and 2% 647-Hilyte tubulin (Cytoskeleton, cat no. TL670M-B) and paclitaxel-stabilized cisplatin resistant (CP-R) microtubules containing 99% CP-R tubulin, 1% biotin brain tubulin were immobilized on a glass surface using neutravidin (Life Technologies, A-2666).

    Techniques: Cell-Signaling, Virus, Activation Assay, Control, Recombinant, Labeling, Mass Spectrometry, Sequencing, Cell Viability Assay, DNA Purification, Amplification, CRISPR, Plasmid Preparation, Synthesized, Software, Analysis, Filtration